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Some patients reach their target weight and choose to stay on semaglutide long-term as a maintenance medication, while others discontinue after significant loss and transition to behavioral strategies

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However, dual immunofluorescence for GLP-1R and the marker for CSF-contacting neurons, PKD2L1 33 , was not feasible, because both antibodies are detected with anti-rabbit secondary IgGs (the GLP-1R antibody is a rabbitized mouse antibody)

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