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This review will provide comprehensive guidance and critical insights to researchers in both industry and academia who are involved in the design of advanced, functional biochemically cleavable conjugations

1 Metabolic diseases like obesity and overweight have traditionally been treated with combinations of behavioral therapy and pharmacotherapy, the latter of which was often associated with poor efficacy and weight regain after medication cessation

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Macrophage production was initiated on day 10 by transferring the aggregated myeloid cell forming complexes onto an adherent 6-well tissue culture plate (TPP) and medium was changed to X-VIVO 15 (Lonza) containing 2 U/mL penicillinstreptomycin, 2 mM L-glutamine, 50 M -mercaptoethanol (all Gibco) supplemented with 25 ng/mL hIL-3 and 50 ng/mL human macrophage colony stimulating factor (hM-CSF, PeproTech)