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The dried plant samples (0.1 g) were mixed with 1.5 ml of methanol, 0.1 ml of potassium acetate, 0.1 ml of 10% AlCl 3 , and 2.8 ml of water

Signals were detected using an HRP-conjugated secondary antibody and the SuperSignal West Femto Maximum Sensitivity Substrate (Pierce, Thermo Fisher Scientific, Inc., Rockford, IL, USA)

Cancer 120 , 175180 (2007)