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The resulting supernatants were vacuum-dried at 37 C, resuspended in 100 L of 0.1% FA, and filtered with 0.2 m RC syringe filter before injection

In a physical dependence assay (assessed at 6, 14, 42, and 72 hours after the conditioning treatment) shorter pre-convulsive latencies were noted in diazepam conditioned mice than in non-conditioned healthy mice following an isoniazid treatment forty-two and seventy-two hours after the conditioning treatment

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