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Cells were lysed in NP40 buffer and protein was quantified using Pierce BCA Protein Assay Kit (Sigma)

Brezova V, Valko M, Breza M, Morris H, Telser J, Dvoranova D, Kaiserova K, Varecka L, Mazur M, Leibfritz D (2003) Role of radicals and singlet oxygen in photoactivated DNA cleavage by the anticancer drug camptothecin: an electron paramagnetic resonance study

It has been explored in early laboratory and preclinical research for its role in cellular signaling and biological processes related to tissue maintenance