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Here, protamine was conjugated to a human IgG-Fc, targeting the (FcRn), known to mediate the transcytosis of IgG across epithelial cells (see Section 6.1.1)

The cDNA for SLC7A11 coding region was amplified from ZR-75-1 breast cancer cell line using standard RT-PCR and ligated into EcoR I- Sal I linearized retroviral pBabe-hygro vector, generating the pBabe-hygro-SLC7A11

doi: 10.1016/j.ebiom.2015.11.025 92

Focus on increasing protein intake to 1.0 to 1.2 grams per kilogram of body weight, as this becomes critical later when appetite suppression makes eating more difficult