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gRNA sequences (Supplementary Table 33) were obtained from Synthego and oligos with 5 overhang for cloning into lentiCRISPRv2 were manufactured by Thermo Fisher Scientific

Obesity & ROSE-010: The Future of Weight Loss Maintenance The GLP-1 Dilemma: Maintaining Weight Loss & Seeking Alternative Weight Loss Options GLP-1 therapies have helped millions lose weight, yet 60-85% of patients discontinue within two years due to: Tolerability Issues Over 50% of GLP-1 patients stop due to nausea, vomiting, or GI side effects Insurance & Cost Barriers Insurance coverage remains inconsistent, limiting long-term access Reaching Their Goa l Many patients lose their desired weight and no longer need daily or weekly injections The Problem