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Reconstituted peptides should be refrigerated at 28C (3646F) and used within 2830 days

Triglycerides levels were determined from the supernatants using the Triglyceride Liquid Assay (Sentinel Diagnostics) following manufacturers instructions as in ( 2.6 Gene expression RNA was extracted from frozen liver tissues using the RNeasy mini kit (Qiagen), treated with DNase I (Sigma-Aldrich), then reverse-transcribed with M-MLV reverse transcriptase (Promega)

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Reactive oxygen disrupts mitochondria in MA-10 tumor Leydig cells and inhibits steroidogenic acute regulatory (StAR) protein and steroidogenesis