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Although folic acid absorption by the intestine is catalyzed by the proton-coupled folate transporter (PCFT), which has similar affinity for both folic acid and reduced folates ( Table 2 ) (65, 66), the affinity for folic acid of the reduced folate carrier (RFC), which is ubiquitously expressed in all tissues, is 50- to 150-fold lower than that for 5-methylTHF and other reduced folates (Table 2) (65, 66)

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2 mL or 3 mL is commonly used in research reconstitution