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Changes in resorufin fluorescence were measured in a 96-well microtiter plates following the protocol outlined in the commercially available Cytochrome P450 2B Fluorescent Detection Kit (CYTO2B

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The perimeter of each square wound was traced onto a sterile piece of clear acetate film by an examiner wearing a 2.5x loupe

Research protocols may examine whether interventions that modulate the glutathione synthesis pathway can affect neuronal survival, protein aggregation (e.g., alpha-synuclein or amyloid-beta), or mitochondrial function in these non-human systems