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The acquired 36000 x magnification (n=39 and n=36 for NbALFA-Gold and NbLumSyt1-ALFA-tag:NbALFA-Gold complex, respectively) and 57000 x magnification tomograms (n=11 for NbLumSyt1-ALFA-tag:NbALFA-Gold complex) were analyzed using 3dmod software and additional programs within the IMOD software package 75

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