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The assay plate was incubated for 3 hours in a 5% CO 2 incubator at 37 C and then rested at RT for 15 minutes before steadylite plus reagent (Perkin Elmer) was added to each well of the assay plate (100 l/well) followed by 30 minutes slowly shaking at RT protected from light

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Conversely, steroid hormones, transforming growth factor- (TGF-), plasma proteins such as a2-macroglobulin and a1-antitrypsin, and tissue inhibitors of metalloproteinases (TIMPs) can inhibit MMP activity [16]

adults had heard of GLP-1 drugs, up from 70 percent in a previous NORC survey conducted in May 2024