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In brief, 500 cells were seeded per well in 384-well plates, and after 24 h, the medium was switched using a series of 4 aspirationwash steps with the desired final medium

This design choice introduces potential bias, particularly for subjective outcomes, but it reflects the practical reality that these treatments have different injection devices and titration schedules that would make blinding extremely difficult

B12 thins the blood and prevents blood stickiness