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Flow cytometry MenSCs were harvested and incubated with antibodies against surface marker and isotype control, including CD29 (BD, #561795), CD73 (BD, #561014), CD90(BD, #561970), CD105 (BD, #560839), CD34 (BD, #560941), CD45 (BD, #560975), CD117 (BD, #561682), HLA-DR (BD, #560943), IgG1 (BD, #555749), and IgG2a (BD, #555574) in the dark at 4 C for 30 min

Continuous medical check-ups are necessary to monitor for manage any long-term risks

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According to the 2018 China Medical Expense Statistics, medical expenses associated with overweight and obesity represented 3.9% of total medical care costs and 4.31% of non-hospitalization expenditures [4]