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In young and aged rats, treadmill exercise for a duration of 6 weeks led to increased SIRT1 activity, NAMPT and NAD + levels, and decreased mitochondrial generation of ROS 302
Since our proteomic study suggested mitochondrial alterations following HC treatment, and considering the recent hypothesis of Simeone (Simeone, 2025) that HC might interact with key cellular antioxidant systems, mitochondrial function was further investigated through oxygen consumption rate (OCR), extracellular acidification rate (ECAR), mitochondrial membrane potential, mitochondrial network morphology, ROS production and GSH/GSSG ratio
They may also affect the activity of phase I biotransformation enzymes like cytochrome P450 (CYP) 3A4 (CYP3A4) (161, 162)
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