For the first time in history, high school athletes are training more like pros than kids multi-sport schedules, club travel, off-season showcases, strength training, nutrition expectations, recruiting pressure, long commutes, poor sleep, and the increasing belief that more is better. Their biology is being asked to mature faster than their endocrine system, and their recovery capacity isnt built for the volume they endure
BD Biosciences, USA) for 30 min, washed with fresh 0.3% BSA, and applied to flow cytometry analysis on an LSRFortessa flow cytometer (BD Biosciences, USA) to count at least 10 000 events
2009;5:e1000472 Ginn J, Sacco J, Wong YY, Motsinger-Reif A, Chun R, Trepanier LA
10.1007/s10787-017-0358-8 [DOI] [PubMed] [Google Scholar] Berkopic Cesar L., Gojkovic S., Krezic I., Malekinusic D., Zizek H., Batelja Vuletic L., et al