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This process is driven by the accumulation of ROS and the peroxidation of polyunsaturated fatty acids in cellular membranes, leading to the formation of lipid hydroperoxides that compromise membrane integrity and cause cell swelling and death

Following isolation and quantification, mitochondria were divided into at least 100 g replicates in microtubes and centrifuged at 11,800 g for 10 min to pellet and were used for respiration and NAD + uptake assays or flash-frozen for metabolite analyses, including NAD + , NADH and metabolomics

Concentrated urine due to dehydration appears darker yellow or amber, whilst adequate hydration produces pale straw-coloured urine