Freezer storage at minus twenty degrees provides significantly better protection than refrigeration at four degrees
These experiments indicated that, in addition to requiring Cys-dependent de novo biosynthesis of GSH for survival (see above), TrxR1/Gsr-null livers require de novo biosynthesis of Cys via transsulfuration, and that they cannot effectively utilize cystine as a hepatic Cys source [107]
10.3389/fimmu.2024.1413485 Front
For instance, the MitoSOX Red probe (Invitrogen, Waltham, MA, USA) can be used to measure oxidative stress localized in the mitochondria, whereas the CellROX Green (Invitrogen, Waltham, MA, USA) and Deep Red probes (Invitrogen, Waltham, MA, USA) can be used to detect intracellular ROS