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3rd & Brookeman, J

GGT and GCL activity was determined through GGT-Activity Colorimetric Assay Kit (Merck Sigma-Aldrich) and GCL Assay Kit (Real-Gene Labs) respectively, following the protocol detailed by the manufacturer

We use high shear homogenization and employ iterative quality, potency, and particle size testing processes using light scattering technology with each and every batch to ensure the smallest particle size to ensure the smallest particle size for maximum absorption

This procedure involved removing the supernatant and resuspending the mitochondrial pellet in an isolation buffer of 250 mM sucrose, 0.5 mM EDTA, and 10 mM Tris-HCl to achieve a final protein concentration of 20-25 mg/ml [2]