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Standard or sample was transferred in triplicate to a 96-well plate and GSH was then derivitized by the addition of 10 mM 2,3-naphthalenedicarboxyaldehyde and the fluorescence at Ex/Em wavelength of 472/528 nm was quantified using a fluorescent plate reader (Synergy 2, BioTek)

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below Properties of Convex and Concave Functions Some common properties related to Concave and Convex Functions are: First Derivative Test for Convexity/Concavity A function f(x) is convex, If f(x) is non-decreasing i , we have: f(\lambda x_1 + (1-\lambda) x_2) \leq \lambda f(x_1) + (1-\lambda) f(x_2) Consider \( f(x) = x^2 \), then: f(\lambda x_1 + (1-\lambda) x_2) = (\lambda x_1 + (1-\lambda) x_2)^2 Expanding this expression: (\lambda x_1 + (1-\lambda) x_2)^2 = \lambda^2 x_1^2 + 2\lambda(1-\lambda)x_1x_2 + (1-\lambda)^2 x_2^2 On the other hand, we have: \lambda f(x_1) + (1-\lambda) f(x_2) = \lambda x_1^2 + (1-\lambda) x_2^2 Now, comparing both sides: \lambda^2 x_1^2 + 2\lambda(1-\lambda)x_1x_2 + (1-\lambda)^2 x_2^2 \leq \lambda x_1^2 + (1-\lambda) x_2^2 Since \ 2\lambda(1-\lambda) x_1 x_2 \geq 0 ,the inequality holds