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Briefly, cells were labelled with 1 M BG-S-S-649, a cleavable SNAP-Surface probe, in full media for 30 minutes at 37C, washed with PBS and stimulated with vehicle or 100 nM exendin-4 in reverse time order for 30, 15, 10 and 5 minutes at 37C in full media

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Conducting three-arm trials that include a placebo group (e.g., distilled water), an L-carnitine group, and a group receiving an equivalent nitrogen dose to the L-carnitine supplement is also suggested