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A temperature gradient was set in the thermocycler for LacAnc100 and LaccID ranging from 30 to 70 C as follows (in C): 30.0, 30.9, 32.7, 35.8, 39.4, 41.8, 43.7, 45.0, 46.8, 49.8, 54.4, 59.9, 64.8, 68.0 and 70.0