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The final 2Fo-Fc electron density map allows for the distinction of carbon, nitrogen and oxygen atoms with temperature factors below about 25 A2

Beyond these direct measures, a culture of vigilance and meticulousness among lab personnel is crucial

Imaging can also be used to look at other organ-specific effects, which the field is beginning to understand better thanks to the various trials that have been conducted and are still ongoing in the heart, liver, kidneys, and to a lesser extent, the brain