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Superoxide reacts with NO to form peroxynitrite (ONOO ), a toxic compound that depletes NO and worsens endothelial dysfunction (Li H
And then, there are factors that increase the need for glutathione like excessive oxidative stress, poor nutritional status, or increased toxic load that will deplete GSH levels if not sufficiently supported and/or replenished 4
Table 1 Common Fusion Tags and Their Properties Tag Hexa-histidine Size (kDa) 0.84 Ligand Ni-IDA Elution Imidazole Properties Small size and easy to integrate into protein, simple purification process, mild elution condition Tag GST Size (kDa) 26 Ligand Glutathione Elution Glutathione Properties Simple purification process, mild elution condition Tag Biotin Size (kDa) 0.244 Ligand Monomeric avidin Elution Biotin Properties Small size, high specificity and affinity to avidin, simple purification process, mild elution condition Size (kDa) Ligand Elution Properties Hexa-histidine 0.84 Ni-IDA Imidazole Small size and easy to integrate into protein, simple purification process, mild elution condition GST 26 Glutathione Glutathione Simple purification process, mild elution condition Biotin 0.244 Monomeric avidin Biotin Small size, high specificity and affinity to avidin, simple purification process, mild elution condition A polyhistidine tag, also called 6xHis-tag, His-tagged, and His-tag is a versatile tool for purifying the highly purified recombinant protein from various expression systems, including bacterial, yeast, plant cell, and mammalian cells systems
