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Although it's uncommon, vitamin B12 or folate deficiency (with or without anaemia) can lead to complications, mainly if you have been deficient in vitamin B12 or folate for some time

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For example, in pernicious anemia (a major cause), antibodies attack the intrinsic factor (IF), a protein required for the absorption of B12, or the gastric parietal cells, which produce IF in the first place

The cDNA was then fourfold diluted and used as a template in quantitative PCR with IQ SYBR Green Supermix (Bio-Rad) on a CFX96 instrument (Bio-Rad) using a 3-step protocol