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A commonly used ADC linker makes use of a valine-citrulline (vc) dipeptide, first discovered by Dubowchik et al., as an intracellular cleavage mechanism by cathepsin B

Preparation Condition 4 (EDTA 4 o C deproteinization): Storage temperature affects total GSH, free GSH, and GSSG, but not the ratio of free GSH to GSSG in plasma We included EDTA-treated plasma stored at 4 o C (Preparation Condition 4) as a positive control for enzymatic degradation of glutathione which may occur during sample storage

Additionally, peptide DSIP has been shown to interact with the hypothalamic-pituitary-adrenal (HPA) axis, which plays a crucial role in stress adaptation and immune function

AIDS 27 , 15131516 (2013)