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Open questions in the GHK-Cu field include the specific cellular targets responsible for the broad transcriptomic effects, the relationship between the copper-delivery role and the direct peptide-only effects (if any), the comparative biology of GHK-Cu and related copper-peptides in defined cellular contexts, and the translation of cell-culture and animal-model findings to clinical-research endpoints under controlled study conditions

Its not a true GLP-1 but a dual GLP-1/glucose-dependent insulinotropic polypeptide (GIP) receptor agonist

Proline residues are known to be structure-breaking [ clarification needed ] residues, so these were directly grafted into the human sequence

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