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Real-time quantitative polymerase chain reaction (RT-qPCR) The samples were added into Trizol for cracking, then chloroform was added for centrifugation to obtain the upper liquid phase, and isopropyl alcohol was added for centrifugation to obtain the precipitate

They are also eating less variety

As early as 1906 Benjamin Moore (figure 8) and his colleagues at the University of Liverpool had postulated that the duodenum produced a hormone that helped to activate internal secretions in the pancreas

The paper established MOTS-c as an "exercise mimetic" and launched the field of mitochondrial-derived peptide research (Lee C et al., Cell Metab