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These free radicals, while a natural byproduct of metabolism, can cause cellular damage if left unchecked

For measurement of metabolite levels in cells, hepatocytes were harvested by treating with 0.25 % trypsin (Gibco #25200), washing with ice-cold PBS to remove trypsin, suspending released hepatocytes in ice-cold PBS, and centrifuging the cell suspension at 1600 g for 10 min at 4 C to obtain the pelleted cells, which were immediately frozen and stored at 80 C for later analysis

Maybe you find that a half dose is just as effective as a full dose

These are common Herxheimer reaction symptoms and can be extremely uncomfortable to deal with