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Intracardiac perfusion of a physiological buffer (128 mM NaCl, 24 mM NaHCO 3 , 4.2 mM KCl, 2.4 mM NaH 2 PO 4 , 1.5 mM CaCl 2 , 0.9 mM MgSO 4 , pH 7.4, supplemented with 0.1% albumin, 20 mM glutamate, 20 mM cystine and 40 mM glycine), was performed at a physiological rate (0.67 mL/min) for 2 min followed by a 5 min perfusion of the same buffer supplemented with CDNB (1mM)

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