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The integrated dataset was then clustered, and highly upregulated genes in each cluster (identified by the Wilcoxon rank sum test in Seurat), along with the spatial context of the clusters location in situ, were used to annotate the predominant cell type in each spot

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doi: 10.1210/en.2010-0068

Ferroptosis is a form of cell death mediated by labile iron and characterized by the unrestricted accumulation of peroxidized membrane phospholipids, leading to membrane damage 10,11