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Cells were washed with PBS, fixed (3.7% formaldehyde/PBS, 20 min, room temperature), washed twice with PBS and permeabilized (0.1% Triton-X100/PBS, 20 min, room temperature)

The antipsychotic activity of phenothiazines may be related to any or all of these effects, but it has been suggested that the drugs' effects on dopamine are probably most important

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