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The absorbance was measured using a microplate reader (Molecular Device, Menlo Park, CA, USA) at a wavelength of 490 nm

Possible complications heart failure severe neurological deficits gastric cancer type 1 diabetes rheumatoid arthritis myasthenia gravis (a chronic condition causing muscle weakness) Hashimotos disease (an autoimmune condition affecting the thyroid) A pregnant or nursing person with a B12 deficiency When a person receives treatment for a B12 deficiency, they generally recover well and cease to have symptoms, although they may need to continue to use B12 nutritional supplements